Data/hora: 06/06/2024 13:26:39

Provedor de dados: 	5	
País: 	France	
Título: 	Taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes	
Autores: 	Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V.	
Data: 	1998-05	
Ano: 	1998	
Palavras-chave: 	TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization.	
Resumo: 	The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No positive hybridization was obtained with other shrimp viruses tested [Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) and Hepatopancreatic Parvovirus (HPV)]. The specificity of the 2 probes was con firmed by in situ hybridization on histological sections of TS diseased shrimps.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00447/55899/57914.pdf

DOI:10.3354/dao033011

http://archimer.ifremer.fr/doc/00447/55899/	
Editor: 	Inter-research	
Formato: 	application/pdf	
Fonte: 	Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1998-05 , Vol. 33 , N. 1 , P. 11-17	
Fonte: 	Inter-Research 1998

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