Data/hora: 13/06/2024 05:26:04

Provedor de dados: | 5
País: | France
Título: | Devenir des virus entériques humains en milieu marin : Apport des VLPs (Virus Like Particles) pour la purification des coquillages
Autores: | Loisy, Fabienne.
Data: | 2004-11-05
Ano: | 2004
Palavras-chave: | Specific binding; Persistence; Shellfish; Real time RT PCR; VLPs; Rotavirus; Norovirus; Fixation spécifique; Persistance; Coquillage; RT PCR en temps réel; VLPs; Rotavirus.
Resumo: | This work was developed following two research axes: the improvement of norovirus (human and animal strain) and rotavirus detection, and the evaluation of VLPs as a viral substitute for studying their comportment in marine environment. The main point concerning the method optimisation was the development of real-time RT-PCR for norovirus detection. The second part of the work has demonstrated the potential of norovirus and rotavirus VLPs as a viral surrogate as proven by the study of their stability in seawater. As VLPs are non-pathogen particles, the long persistence of rotavirus VLPs in shellfish has been shown in natural conditions (oysters on marine estuaries). The studies of Norwalk virus VLPs attachment to oyster tissues have demonstrated a specific binding to some tissues.
Tipo: | Text
Idioma: | Francês
Identificador: | http://archimer.ifremer.fr/doc/2004/these-327.pdf
Editor: | Université Paris XI
Relação: | http://archimer.ifremer.fr/doc/00000/327/
Formato: | application/pdf