Data/hora: 10/06/2024 19:08:14

Provedor de dados: | 5
País: | France
Título: | Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos
Autores: | Diter, Alain; Dufy, Christian.
Data: | 1990-06
Ano: | 1990
Palavras-chave: | Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B.
Resumo: | Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/1990/publication-2753.pdf
Editor: | EDP Sciences
Relação: | http://archimer.ifremer.fr/doc/00000/2753/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (EDP Sciences), 1990-06 , Vol. 3 , N. 2 , P. 107-112
Fonte: | EDP Sciences