Data/hora: 06/06/2024 18:09:56

Provedor de dados: | 5
País: | France
Título: | Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas
Autores: | Perez Garcia, Maria Concepcion; Rouxel, Julien; Akcha, Farida.
Data: | 2015-04
Ano: | 2015
Palavras-chave: | Crassostrea gigas; Hydrogen peroxide; Comet assay; Comet-FISH; Genotoxicity.
Resumo: | In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the number of signals present in comet tails. This study demonstrates the potential value of the comet-FISH assay for the study of DNA damage induced by genotoxicant exposure of target genes. It offers a perspective for better understanding the impact of genotoxicity on animal physiology and fitness.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00250/36157/34712.pdf

DOI:10.1016/j.aquatox.2015.01.022

https://archimer.ifremer.fr/doc/00250/36157/

Editor: | Elsevier Science Bv
Formato: | application/pdf
Fonte: | Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2015-04 , Vol. 161 , P. 189-195

Fonte:

2015 Published by Elsevier B.V.

info:eu-repo/semantics/openAccess

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