Data/hora: 15/06/2024 02:45:07

Provedor de dados: | 5
País: | France
Título: | Differential gene expression in Litopenaeus vannamei shrimp in response to diet changes
Autores: | Chavez-calvillo, G.; Perez-rueda, E.; Lizama, G.; Zuniga Aguilar, J. J.; Gaxiola, G.; Cuzon, Gerard; Arena-ortiz, L.
Data: | 2010-02
Ano: | 2010
Palavras-chave: | Differential display; RT-PCR; Litopenaeus vannamei; Protein diet; Alternative protein sources.
Resumo: | We evaluated changes in gene expression of Litopenaeus vannamei shrimp in response to a change in diet by comparing specimens which were fed with an animal protein diet against those which underwent a change from an animal protein diet to a plant protein diet. In addition, we also compared specimens which were fed with a plant protein diet with another group which underwent a change from plant to animal protein diet. Results showed that, in the case of hepatopancreas tissue, a total of six genes were differentially expressed and exhibited a high degree of homology with sequences which codify for proteins related to metabolism, transcription regulation, and rRNA subunits. In the case of muscle tissue, four differentially expressed sequences were recorded which codified for proteins associated to the immune system response, as well as cell structure and signaling. By means of RT-PCR, we also detected differentially expressed chitinase transcripts (Delta Ct). We observed Delta Ct expression values of 0.49 when comparing specimens fed a plant diet to those which underwent a change from plant to an animal diet, while a Delta Ct value of 0.52 was observed when comparing animals fed with an animal diet to specimens which underwent a change from an animal to a plant protein diet Differential gene expression was not observed in specimens which did not undergo a change in diet during the experiment.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00002/11298/7853.pdf

DOI:10.1016/j.aquaculture.2009.11.027

Editor: | Elsevier Science Bv
Relação: | http://archimer.ifremer.fr/doc/00002/11298/
Formato: | application/pdf
Fonte: | Aquaculture (0044-8486) (Elsevier Science Bv), 2010-02 , Vol. 300 , N. 1-4 , P. 137-141
Fonte: | 2010 Elsevier B.V. All rights reserved.