Data/hora: 06/06/2024 12:07:56

Provedor de dados: | 5
País: | France
Título: | Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon
Autores: | Leis, Benedikt; Heinze, Simon; Angelov, Angel; Pham, Vu Thuy Trang; Thürmer, Andrea; Jebbar, Mohamed; Golyshin, Peter N.; Streit, Wolfgang R.; Daniel, Rolf; Liebl, Wolfgang.
Data: | 2015-07
Ano: | 2015
Palavras-chave: | Functional screenings; Extreme thermostable protein; Archaeal endoglucanase; Enzymatic characterization.
Resumo: | Extreme habitats serve as a source of enzymes that are active under extreme conditions and are candidates for industrial applications. In this work, six large-insert mixed genomic libraries were screened for hydrolase activities in a broad temperature range (8–70°C). Among a variety of hydrolytic activities, one fosmid clone, derived from a library of pooled isolates of hyperthermophilic archaea from deep sea vents, displayed hydrolytic activity on carboxymethyl cellulose substrate plates at 70°C but not at lower temperatures. Sequence analysis of the fosmid insert revealed a gene encoding a novel glycoside hydrolase family 12 (GHF12) endo-1,4-β-glucanase, termed Cel12E. The enzyme shares 45% sequence identity with a protein from the archaeon Thermococcus sp. AM4 and displays a unique multidomain architecture. Biochemical characterization of Cel12E revealed a remarkably thermostable protein, which appears to be of archaeal origin. The enzyme displayed maximum activity at 92°C and was active on a variety of linear 1,4-β-glucans like carboxymethyl cellulose, β-glucan, lichenan, and phosphoric acid swollen cellulose. The protein is able to bind to various insoluble β-glucans. Product pattern analysis indicated that Cel12E is an endo-cleaving β-glucanase. Cel12E expands the toolbox of hyperthermostable archaeal cellulases with biotechnological potential.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00601/71319/69744.pdf

https://archimer.ifremer.fr/doc/00601/71319/69745.pdf

https://archimer.ifremer.fr/doc/00601/71319/69746.pdf

DOI:10.3389/fbioe.2015.00095

https://archimer.ifremer.fr/doc/00601/71319/

Editor: | Frontiers Media SA

Relação:

info:eu-repo/grantAgreement/EC/FP7/287589/EU//MICRO B3

info:eu-repo/grantAgreement/EC/FP7/226977/EU//MAMBA

Formato: | application/pdf
Fonte: | Frontiers in Bioengineering and Biotechnology (2296-4185) (Frontiers Media SA), 2015-07 , Vol. 3 , N. 95 , P. 10p.

Fonte:

info:eu-repo/semantics/openAccess

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