Data/hora: 06/06/2024 13:34:55

Provedor de dados: 	5	
País: 	France	
Título: 	Preparation techniques alter the mineral and organic fractions of fish otoliths: insights using Raman micro-spectrometry	
Autores: 	Jolivet, Aurelie; Fablet, Ronan; Bardeau, Jean-francois; De Pontual, Helene.	
Data: 	2013-05	
Ano: 	2013	
Palavras-chave: 	Epoxy resin; Otolith composition; Microstructure; Raman spectrometry.	
Resumo: 	The high spatial resolution analysis of the mineral and organic composition of otoliths using Raman micro-spectrometry involves rigorous protocols for sample preparation previously established for microchemistry and trace elements analyses. These protocols often include otolith embedding in chemically neutral resin (i.e., resins which do not contain, in detectable concentration, elements usually sought in the otoliths). Such embedding may however induce organic contamination. In this paper, Raman micro-spectrometry reveals the presence of organic contamination onto the surface obtained from the use of epoxy resin, specifically Araldite. This contamination level varies depending on otolith structures. Core and checks, known as structural discontinuities, exhibit the most important level of contaminations. Our results suggest that otolith embedding with resin affects the organic matrix of the otolith, probably through an infiltration of the resin in the crystalline structure. The interpretation of chemical otolith signatures, especially Raman otolith signatures, and stable isotope analyses should then be revised in light of these results. In this respect, we propose a method for the correction of Raman otolith signatures for contamination effects.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00138/24965/26372.pdf

DOI:10.1007/s00216-013-6893-2	
Editor: 	Springer Heidelberg	
Relação: 	http://archimer.ifremer.fr/doc/00138/24965/	
Formato: 	application/pdf	
Fonte: 	Analytical And Bioanalytical Chemistry (1618-2642) (Springer Heidelberg), 2013-05 , Vol. 405 , N. 14 , P. 4787-4798	
Fonte: 	Springer-Verlag Berlin Heidelberg 2013