Data/hora: 01/06/2024 10:21:57

Provedor de dados: 	5	
País: 	France	
Título: 	Innate Immune Responses of a Scleractinian Coral to Vibriosis	
Autores: 	Vidal-dupiol, Jeremie; Ladriere, Ophelie; Destoumieux-garzon, Delphine; Sautiere, Pierre-eric; Meistertzheim, Anne-leila; Tambutte, Eric; Tambutte, Sylvie; Duval, David; Foure, Laurent; Adjeroud, Mehdi; Mitta, Guillaume.	
Data: 	2011-06	
Ano: 	2011	
Resumo: 	Scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are among the most diverse of all ecosystems. Over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographically widespread and a major threat to these ecosystems. Although coral immunity has been the subject of increasing study, the available information remains fragmentary, especially with respect to coral antimicrobial responses. In this study, we characterized damicornin from Pocillopora damicornis, the first scleractinian antimicrobial peptide (AMP) to be reported. We found that its precursor has a segmented organization comprising a signal peptide, an acidic proregion, and the C-terminal AMP. The 40-residue AMP is cationic, C-terminally amidated, and characterized by the presence of six cysteine molecules joined by three intramolecular disulfide bridges. Its cysteine array is common to another AMP and toxins from cnidarians; this suggests a common ancestor, as has been proposed for AMPs and toxins from arthropods. Damicornin was active in vitro against Gram-positive bacteria and the fungus Fusarium oxysporum. Damicornin expression was studied using a combination of immunohistochemistry, reverse phase HPLC, and quantitative RT-PCR. Our data show that damicornin is constitutively transcribed in ectodermal granular cells, where it is stored, and further released in response to nonpathogenic immune challenge. Damicornin gene expression was repressed by the coral pathogen Vibrio coralliilyticus. This is the first evidence of AMP gene repression in a host-Vibrio interaction.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00039/14996/12433.pdf

DOI:10.1074/jbc.M110.216358	
Editor: 	Amer Soc Biochemistry Molecular Biology Inc	
Relação: 	http://archimer.ifremer.fr/doc/00039/14996/	
Formato: 	application/pdf	
Fonte: 	Journal Of Biological Chemistry (0021-9258) (Amer Soc Biochemistry Molecular Biology Inc), 2011-06 , Vol. 286 , N. 25 , P. 22688-22698	
Fonte: 	2011 by The American Society for Biochemistry and Molecular Biology, Inc.