Data/hora: 06/06/2024 12:08:10

Provedor de dados: | 5
País: | France
Título: | Etude des facteurs de transcription impliqués dans l’accumulation lipidique en condition de stress azoté chez la microalgue haptophyte Isochrysis affinis galbana
Autores: | Thiriet-rupert, Stanislas.
Data: | 2017-01-10
Ano: | 2017
Palavras-chave: | Bioinformatique; Biologie moléculaire; Évolution; Facteur de transcription; Micro-algue; Réseau de gènes; RNA-seq; Bioinformatic; Evolution; Gene network; Molecular biology; Microalgae; RNAseq; Transcription factor.
Resumo: | In every organism, evolution and acclimation to environmental changes are orchestrated by numerous molecular players. Among them, transcription factors (TFs) play a crucial role by regulating gene expression. Therefore, identify TFs involved in the production of high value products is a significant step in a biotechnological context. The laboratory has at its disposal a mutant strain of the haptophyte microalga Tisochrysis lutea producing twice more storage lipids than the wild type strain when exposed to nitrogen deprivation. Given the key role of TFs in phenotype establishment, this PhD aim at identify the TFs involved in that of the mutant phenotype of T. lutea. A TFs identification and classification pipeline was elaborated and applied to T. lutea’s genome. Since the lack of data in haptophytes constitutes a limit in studies on microalgae evolutionary history, a comparative study of TFs identified in the genome of microalgae belonging to different lineages was carried out. This study reveals that TFs could be used to understand and illustrate microalgae evolutionary history through the highlight of lineage specific presence/absence of TF families. Aiming at understanding T. lutea’s mutant strain phenotype establishment, transcriptomic data were used to build gene co-expression networks and gene regulatory networks for both strains. Their comparative analysis identified seven TFs potentially liked to the mutant phenotype. A q-RT-PCR approach confirmed the involvement of two TFs in nitrogen recycling under nitrogen deprivation.
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00371/48233/48368.pdf

http://archimer.ifremer.fr/doc/00371/48233/

Editor: | Université du Maine
Formato: | application/pdf

Fonte:

2017 Université du Maine

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