Data/hora: 06/06/2024 13:47:06

Provedor de dados: 	5	
País: 	France	
Título: 	Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass (Dicentrarchus labrax): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance	
Autores: 	Ollivier, Helene; Marchant, James; Le Bayon, Nicolas; Servili, Arianna; Claireaux, Guy.	
Data: 	2015-10	
Ano: 	2015	
Palavras-chave: 	Sea bass; Myocyte populations; Calcium signaling; Cardiac plasticity; Inter-individual variability.	
Resumo: 	Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22 A degrees C and changes in intracellular calcium concentration ([Ca2+](i)) following KCl stimulation were measured using Fura-2, at 12 or 22 A degrees C-test. The increase in [Ca2+](i) resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22 A degrees C-acclimated fish. In particular, a greater increase in [Ca2+](i) at a high level of adrenaline was observed in 22 A degrees C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes' capacity for environmental adaptation.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00283/39412/37904.pdf

DOI:10.1007/s00360-015-0924-6

https://archimer.ifremer.fr/doc/00283/39412/	
Editor: 	Springer Heidelberg	
Formato: 	application/pdf	
Fonte: 	Journal Of Comparative Physiology B-Biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2015-10 , Vol. 185 , N. 7 , P. 755-765	
Fonte: 	Springer-Verlag Berlin Heidelberg 2015

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