Data/hora: 11/06/2024 04:20:23

Provedor de dados: | 5
País: | France
Título: | Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla)
Autores: | Peruzzi, Stefano; Chatain, Beatrice; Menu, Bruno.
Data: | 2005
Ano: | 2005
Palavras-chave: | Anguilla anguilla; Liza ramada; Sparus aurata; Dicentrarchus labrax; Teleosts; DNA content.
Resumo: | The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus (+/- CI) was 1.55 +/- 0.02 pg for D. labrax, 1.90 +/- 0.03 pg for S. aurata, 1.57 +/- 0.02 pg for L. ramada, and 2.43 +/- 0.04 pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between males and females in either D. labrax or L. ramada.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2005/publication-411.pdf

DOI:10.1051/alr:2005008

Editor: | EDP Sciences
Relação: | http://archimer.ifremer.fr/doc/00000/411/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (EDP Sciences), 2005 , Vol. 18 , N. 1 , P. 77-81
Fonte: | EDP Sciences, IFREMER, IRD 2005