Data/hora: 01/06/2024 03:27:01

Provedor de dados: 	5	
País: 	France	
Título: 	Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var)	
Autores: 	Green, Timothy; Montagnani, Caroline.	
Data: 	2013-08	
Ano: 	2013	
Palavras-chave: 	Crassostrea gigas; OsHV-1; Poly I:C; Vibrio splendidus; Interferon-like response.	
Resumo: 	In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection against subsequent viral infection. In both experiments, genes homologous to a toll-like receptor (TLR), MyD88, interferon regulatory factor (IRF) and protein kinase R (PKR) were up-regulated in oysters immune-primed with poly I:C compared to seawater controls (p &lt; 0.05). The MyD88, IRF and PKR genes were also significantly up-regulated in response to OsHV-1 mu var infection (p &lt; 0.05), which is suggestive that they are implicated in the antiviral response of C gigas. Our results demonstrate that C gigas can recognise double-strand RNA to initiate an innate immune response that inhibits viral infection. The observed response has striking similarities to the hallmarks of the type-1 interferon response of vertebrates.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00152/26293/24739.pdf

DOI:10.1016/j.fsi.2013.04.051	
Editor: 	Academic Press Ltd- Elsevier Science Ltd	
Relação: 	http://archimer.ifremer.fr/doc/00152/26293/	
Formato: 	application/pdf	
Fonte: 	Fish &amp; Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2013-08 , Vol. 35 , N. 2 , P. 382-388	
Fonte: 	2013 Elsevier Ltd. All rights reserved.