Data/hora: 01/06/2024 07:23:38

Provedor de dados: | 5
País: | France
Título: | Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var)
Autores: | Green, Timothy; Montagnani, Caroline.
Data: | 2013-08
Ano: | 2013
Palavras-chave: | Crassostrea gigas; OsHV-1; Poly I:C; Vibrio splendidus; Interferon-like response.
Resumo: | In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection against subsequent viral infection. In both experiments, genes homologous to a toll-like receptor (TLR), MyD88, interferon regulatory factor (IRF) and protein kinase R (PKR) were up-regulated in oysters immune-primed with poly I:C compared to seawater controls (p &lt; 0.05). The MyD88, IRF and PKR genes were also significantly up-regulated in response to OsHV-1 mu var infection (p &lt; 0.05), which is suggestive that they are implicated in the antiviral response of C gigas. Our results demonstrate that C gigas can recognise double-strand RNA to initiate an innate immune response that inhibits viral infection. The observed response has striking similarities to the hallmarks of the type-1 interferon response of vertebrates.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00152/26293/24739.pdf

DOI:10.1016/j.fsi.2013.04.051

Editor: | Academic Press Ltd- Elsevier Science Ltd
Relação: | http://archimer.ifremer.fr/doc/00152/26293/
Formato: | application/pdf
Fonte: | Fish &amp; Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2013-08 , Vol. 35 , N. 2 , P. 382-388
Fonte: | 2013 Elsevier Ltd. All rights reserved.