Data/hora: 05/06/2024 02:25:17

Provedor de dados: | 5
País: | France
Título: | Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum
Autores: | Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compere, Chantal.
Data: | 2012-07-04
Ano: | 2012
Palavras-chave: | Alexandrium Minutum; Avidin; Biosensor; Biotin; DNA; Quartz crystal miocrobalance; Self-assembled monolayer; Thiol.
Resumo: | Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00107/21810/19385.pdf

DOI:10.3390/bios2030245

Editor: | MDPI
Relação: | http://archimer.ifremer.fr/doc/00107/21810/
Formato: | application/pdf
Fonte: | Biosensors (2079-6374) (MDPI), 2012-07-04 , Vol. 2 , N. 3 , P. 245-254
Fonte: | 2012 by the authors; licensee MDPI, Basel, Switzerland