Data/hora: 06/06/2024 08:16:27

Provedor de dados: | 5
País: | France
Título: | Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions
Autores: | Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno.
Data: | 1998-11
Ano: | 1998
Palavras-chave: | Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation.
Resumo: | A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P &lt; 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P &lt; 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied. When 70.10(3) and 200.10(3) spermatozoa per egg were provided in the same experimental conditions, no significant difference appeared between the fertilisation rates of fresh arid thawed sperm. In order to validate the procedure for production or cryobank purpose, a scaled-up protocol was established. Two and 50 mL batches of eggs (approximately 2.10(3) and 50.10(3) eggs, respectively) were inseminated in triplicate using either fresh or-thawed individual sperms of 5 males with 200.10(3) spermatozoa per egg. The mean fertility decreased by 23.5% due to cryopreservation. This decline was explained by the loss of fertility of only one sperm, and only in large-volume conditions, probably due to the delay of use after thawing.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/1998/publication-866.pdf

DOI:10.1016/S0990-7440(99)80004-7

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/866/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (Elsevier), 1998-11 , Vol. 11 , N. 6 , P. 387-394
Fonte: | 1998 Published by Elsevier SAS