Data/hora: 02/06/2024 00:39:28

Provedor de dados: | 5
País: | France

Título:

Etude de la biosynthèse des polysaccharides bactériens : vers la production recombinante d'hétéropolysaccharide

Study of a bacterial polysaccharide biosynthesis : towards the recombinant production of heteropolysaccharide

Autores: | Lebellenger, Lou.
Data: | 2017-12-20
Ano: | 2017
Palavras-chave: | Vibrio diabolicus; Polysaccharides; HE800; GAGmimétiques; Biosynthèse; Vibrio diabolicus; Polysaccharides; HE800; GAGmimetics; Biosynthesis.
Resumo: | The marine bacteria Vibrio diabolicus produces an exopolysaccharide (EPS) called HE800. The native form of HE800 has already shown biological efficiency in bone healing. It is studied as glycosaminoglycan-mimetic for applications in cosmetic and human health. To enhance biotechnological applications of polysaccharides, the chemical structures should be properly controlled; moreover, the production yield could also be a bottleneck. The genetic cluster of the HE800 EPS biosynthesis has been identified in 2014. However, the biosynthesis molecular mechanisms and regulations remain sometimes unclear. The main objective of this work is to obtain a recombinant production of the HE800 EPS in the common host Escherichia coli, to improve the knowledge of its biosynthesis mechanisms. During this thesis, a comparative study in Vibrionaceae genomes was conducted to identify EPS biosynthesis genetic clusters similar to the HE800 one; it reveals that this cluster is largely widespread and present in 70 % of the 103 genomes studied. The gene cluster responsible for the HE800 production has been cloned in Escherichia coli. Many tests were performed, but no recombinant production was obtained, showing the complexity of the biosynthesis mechanisms and regulation. These mechanisms were further investigated by transcriptomic analysis of V. diabolicus upon HE800 biosynthesis. In addition, qPCR transcriptional studies of V. diabolicus and of the recombinant strain have highlighted the SypG protein importance in the cluster expression regulation.
Tipo: | Text
Idioma: | Francês

Identificador:

https://archimer.ifremer.fr/doc/00497/60911/64261.pdf

https://archimer.ifremer.fr/doc/00497/60911/

Editor: | Université de Nantes
Formato: | application/pdf

Fonte:

info:eu-repo/semantics/openAccess

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