Data/hora: 06/06/2024 15:29:28

Provedor de dados: | 5
País: | France
Título: | Effecteurs moléculaires de l'association Crassostrea gigas / Vibrio splendidus : role de la porine OmpU dans les mécanismes de résistance et d'échappement à la réponse immunitaire de l'hôte
Autores: | Duperthuy, Marylise.
Data: | 2010-11-04
Ano: | 2010
Resumo: | Vibrio splendidus LGP32 is a bacterial pathogen associated to the summer mortality outbreaks that have affected the production of Crassostrea gigas oysters over the past decades. We showed here that the OmpU porin is a major effector of the V. splendidus / C. gigas interaction. For that, we have constructed a ΔompU mutant of V. splendidus, and shown that the OmpU porin is implicated (i) in the resistance of V. splendidus to antimicrobials, including those of oyster, (ii) in its in vivo fitness, and (iii) in its virulence in oyster experimental infections (mortalities have been reduced from 56 % to 11 % upon mutation). In agreement, we have shown that the ompU deletion modified the expression of secreted proteins controlled by the virulence (ToxR) and the membrane integrity regulation pathways. Furthermore, we have shown that OmpU has a major role in the recognition of V. splendidus by oyster hemocytes. Indeed, (i) in vivo, hemocyte genes displayed differential responses to an infection with the wild-type or the ΔompU mutant, and (ii) in vitro, OmpU was necessary for hemocyte invasion by V. splendidus. This invasion process required the hemocyte integrin and the oyster plasma extracellular SOD, which was found to act as an opsonin recognizing OmpU. Thus, OmpU is a major virulence factor that allows infection of hemocytes in which V. splendidus is able to survive by inhibiting the production of reactive oxygen species and the formation of acidic vacuoles. Resistance of V. splendidus to hemocyte antimicrobials, which is also OmpU-dependant, is probably an additional determinant of V. splendidus intracellular survival.
Tipo: | Text
Idioma: | Francês
Identificador: | http://archimer.ifremer.fr/doc/00028/13905/11071.pdf
Editor: | Université Montpellier 2
Relação: | http://archimer.ifremer.fr/doc/00028/13905/
Formato: | application/pdf
Fonte: | 2010 The author / Univ Montpellier 2