Data/hora: 05/06/2024 04:49:20

Provedor de dados: 	5	
País: 	France	
Título: 	Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss) liver	
Autores: 	Panserat, S.; Hortopan, G. A.; Plagnes-juan, E.; Kolditz, C.; Lansard, M.; Skiba-cassy, S.; Esquerre, D.; Geurden, I.; Medale, Francoise; Kaushik, Sadasivam; Corraze, Genevieve.	
Data: 	2009-09	
Ano: 	2009	
Palavras-chave: 	Rainbow trout; Transcriptome; Metabolism; Liver; Vegetable products; Marine ingredients.	
Resumo: 	Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A large majority of differentially expressed genes were involved in metabolism (57%) and the others in cellular processes (21%) and transport (10%). Among the genes involved in metabolism (n = 86), 37% were associated with protein metabolism (proteolysis, amino acid catabolism), 21% with lipid metabolism (fatty acid biosynthesis, cholesterol biosynthesis), 30% with nucleic acid metabolism and 8% with glucose metabolism. Specifically, we found in rainbow trout fed the 100% plant diet an over-expression of genes involved in lipid biosynthesis (cholesterol metabolism and desaturation of polyunsaturated fatty acids) and an over-expression of a new metabolic actor, i.e., glycerol kinase which plays a key role at the interface of glucose-lipid metabolism. Overall, these data demonstrate that a number of intermediary metabolic effects occur in trout fed a totally plant-based diet. (C) 2009 Elsevier B.V. All rights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2009/publication-6684.pdf

DOI:10.1016/j.aquaculture.2009.05.013	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/6684/	
Formato: 	application/pdf	
Fonte: 	Aquaculture (Elsevier), 2009-09 , Vol. 294 , N. 1-2 , P. 123-131	
Fonte: 	2009 Elsevier Ltd All rights reserved.