Data/hora: 01/06/2024 10:07:59

Provedor de dados: 	5	
País: 	France	
Título: 	Infection dynamics of a V. splendidus strain pathogenic to Mytilus edulis : In vivo and in vitro interactions with hemocytes	
Autores: 	Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank.	
Data: 	2017-11	
Ano: 	2017	
Palavras-chave: 	Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte.	
Resumo: 	The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were successfully produced (16.6% and 45% mortalities in 3 days and 6 days). The amount of GFP Vibrio in seawater decreased during the experiment suggesting its horizontal transfer from diseased animals to healthy ones. At the same time periods, bacteria were detected in hemocytes and in various organs and caused necrosis especially in gills. Total hemocyte count and viability were affected. Taken together, our results indicate that the pathogen V. splendidus 10/068 1T1 colonizes its host both by bypassing external defense barriers and impairing hemocyte defense activities.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00400/51136/53772.pdf

DOI:10.1016/j.fsi.2017.09.047

http://archimer.ifremer.fr/doc/00400/51136/	
Editor: 	Academic Press Ltd- Elsevier Science Ltd	
Formato: 	application/pdf	
Fonte: 	Fish &amp; Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2017-11 , Vol. 70 , P. 515-523	
Fonte: 	2017 Elsevier Ltd. All rights reserved.

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