Data/hora: 03/06/2024 11:26:18

Provedor de dados: | 5
País: | France
Título: | Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas
Autores: | Fabioux, Caroline; Huvet, Arnaud; Lelong, C; Robert, Rene; Pouvreau, Stephane; Daniel, Jean-yves; Mingant, Christian; Le Pennec, M.
Data: | 2004-07
Ano: | 2004
Palavras-chave: | Primordial germ cells; Oyster vasa like gene; Germline; Development; Crassostrea gigas.
Resumo: | The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae body and that would correspond to primordial germ cells (PGCs). Based on these results, we postulate that the germline of C. gigas is specified at early development by maternal cytoplasmic determinants including Oyvlg mRNAs, in putative PGCs that would differentiate into germinal stem cells in juvenile oysters.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2004/publication-648.pdf

DOI:10.1016/j.bbrc.2004.06.009

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/648/
Formato: | application/pdf
Fonte: | Biochemical and Biophysical Research Communications (0006-291X) (Elsevier), 2004-07 , Vol. 320 , N. 2 , P. 592-598
Fonte: | 2004 Elsevier Inc. All rights reserved