Data/hora: 10/06/2024 07:35:28

Provedor de dados: | 5
País: | France
Título: | Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation
Autores: | Zarski, Daniel; Thaovi Nguyen,; Le Cam, Aurelie; Montfort, Jerome; Dutto, Gilbert; Vidal, Marie-odile; Fauvel, Christian; Bobe, Julien.
Data: | 2017-02
Ano: | 2017
Palavras-chave: | Microarray; Genomics; Transcriptomics; Aquaculture; Controlled reproduction.
Resumo: | Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were used. We identified 39 differentially expressed genes exhibiting a differential expression between the groups of low (fertilization rate &lt; 60 %) and high (fertilization rate &gt; 60 %) quality. The mRNA levels of eight genes were further analyzed by quantitative PCR. Seven genes were confirmed by qPCR to be differentially expressed in eggs of low and high quality. This study confirmed the importance of some of the genes already reported to be potential molecular quality indicators (mainly rnf213 and irf7), but we also found new genes (mainly usp5, mem-prot, plec, cenpf), which had not yet been reported to be quality-dependent in fish. These results suggest the importance of genes involved in several important processes, such as protein ubiquitination, translation, DNA repair, and cell structure and architecture; these probably being the mechanisms that contribute to egg developmental competence in sea bass.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00371/48220/48345.pdf

DOI:10.1007/s10126-017-9732-1

http://archimer.ifremer.fr/doc/00371/48220/

Editor: | Springer
Formato: | application/pdf
Fonte: | Marine Biotechnology (1436-2228) (Springer), 2017-02 , Vol. 19 , N. 1 , P. 102-115

Fonte:

The Author(s) 2017. This article is published with open access at Springerlink.com

info:eu-repo/semantics/openAccess

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