Data/hora: 01/06/2024 10:01:30

Provedor de dados: | 5
País: | France
Título: | Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river
Autores: | Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise.
Data: | 2015-04
Ano: | 2015
Palavras-chave: | Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR.
Resumo: | The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature. Increase of DNA adducts after two months of transplantation could be correlated with strong modulation of gene expression implicated in detoxification processes. Indeed, PgP and HSP70 gene expressions were similarly induced in gills and digestive glands while SOD and CAT expressions were down regulated in both tissues. AHR, GST and MT genes were differently regulated depending upon the tissue studied and the level of contamination in the different sites. We demonstrated that mussels transplanted in the different stations with pollution gradient were able to biotransform PAHs, assessed by DNA adduct formation and the high decrease of detoxification genes. Specific DNA adducts pattern obtained after one and two month mussel transplantations demonstrated the relevance of DNA adduct as biomarker of environmental pollution.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00195/30639/32450.pdf

DOI:10.1016/j.ecoenv.2014.03.023

https://archimer.ifremer.fr/doc/00195/30639/

Editor: | Academic Press Inc Elsevier Science
Formato: | application/pdf
Fonte: | Ecotoxicology And Environmental Safety (0147-6513) (Academic Press Inc Elsevier Science), 2015-04 , Vol. 114 , P. 241-249

Fonte:

2014 Elsevier Inc. All rights reserved.

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