Data/hora: 07/06/2024 09:53:22

Provedor de dados: 	5	
País: 	France	
Título: 	Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae	
Autores: 	Mersni-achour, Rachida; Ben Cheikh, Yosra; Pichereau, Vianney; Doghri, Ibtissem; Etien, Cedric; Degremont, Lionel; Saulnier, Denis; Fruitier-arnaudin, Ingrid; Travers, Marie-agnes.	
Data: 	2015-05	
Ano: 	2015	
Resumo: 	Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs causing bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with a LD50 about 2.3 × 103 cfu ml-1 after 24 h. Moreover, complete or GP- HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECPs fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00252/36367/34907.pdf

DOI:10.1099/mic.0.000058

https://archimer.ifremer.fr/doc/00252/36367/	
Editor: 	Soc General Microbiology	
Formato: 	application/pdf	
Fonte: 	Microbiology-sgm (1350-0872) (Soc General Microbiology), 2015-05 , Vol. 161 , P. 997-1007	
Fonte: 	2015 Society for General Microbiology

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