Data/hora: 10/06/2024 08:00:21

Provedor de dados: 	5	
País: 	France	
Título: 	ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas )	
Autores: 	Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.	
Data: 	2017-10	
Ano: 	2017	
Palavras-chave: 	Spermatozoa; Quality; Motility; Viability; ATP content; ROS production.	
Resumo: 	Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability of fertilizing ability demonstrated using simple and multiple regressions. Our results showed that ATP content depends on both MMP and viability of spermatozoa. Using multiple regressions, 61% of the variability of the trochophore-larval yield was explained by a model combining the ATP content and the percentage of dying spermatozoa (P &lt; 0.001). Our results reveal that capacity of spermatozoa to maintain a high level of ATP via OXPHOS partly explains the inter-individual variability of fertilization success in the Pacific oyster. Sperm ATP content and viability assays will provide valuable tools for assessing sperm quality of this species in aquaculture production, cryopreservation, and bioassays.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00386/49769/50309.pdf

DOI:10.1016/j.aquaculture.2017.05.035

http://archimer.ifremer.fr/doc/00386/49769/	
Editor: 	Elsevier Science Bv	
Relação: 	info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED	
Formato: 	application/pdf	
Fonte: 	Aquaculture (0044-8486) (Elsevier Science Bv), 2017-10 , Vol. 479 , P. 114-119	
Fonte: 	2017 Elsevier B.V. All rights reserved.

info:eu-repo/semantics/openAccess

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