Data/hora: 01/06/2024 03:33:07

Provedor de dados: 	5	
País: 	France	
Título: 	Penaeidins, antimicrobial peptides with chitin-binding activity, are produced and stored in shrimp granulocytes and released after microbial challenge	
Autores: 	Destoumieux, Delphine; Munoz, Marcello; Cosseau, Céline; Rodriguez, Jenny; Bulet, Philippe; Comps, Marie-annick; Bachere, Evelyne.	
Data: 	2000-02	
Ano: 	2000	
Palavras-chave: 	Crustacean immunity; Penaeid shrimp; Haemocyte; Chitin binding; Antimicrobial peptide.	
Resumo: 	Penaeidins are members of a new family of antimicrobial peptides isolated from a crustacean, which present both Gram-positive antibacterial and antifungal activities. We have studied the localization of synthesis and storage of penaeidins in the shrimp Penaeus vannamei. The distribution of penaeidin transcripts and peptides in various tissues reveals that penaeidins are constitutively synthesized and stored in the shrimp haemocytes. It was shown by immunocytochemistry, at both optical and ultrastructural levels, that the peptides are localized in granulocyte cytoplasmic granules, The expression and localization of penaeidins were further analysed in shrimp subjected to microbial challenge. We found that (1) penaeidin mRNA levels decrease in circulating haemocytes in the first 3 hours following stimulation and (2) an increase in plasma penaeidin concentration occurs after microbial challenge, together with (3) a penaeidin immunoreactivity in cuticular tissue, which can be related to the chitin-binding activity we demonstrate here for penaeidins.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2000/publication-412.pdf	
Editor: 	Company of Biologists	
Relação: 	http://archimer.ifremer.fr/doc/00000/412/	
Formato: 	application/pdf	
Fonte: 	Journal of Cell Science (0021-9533) (Company of Biologists), 2000-02 , Vol. 113 , N. 3 , P. 461-469	
Fonte: 	The Company of Biologists Limited 2000