Data/hora: 01/06/2024 09:32:25

Provedor de dados: | 5
País: | France
Título: | A novel mcl-PHA produced on coprah oil by Pseudomonas guezennei biovar. tikehau, isolated from a "kopara" mat of French Polynesia
Autores: | Simon-colin, Christelle; Raguenes, Gerard; Crassous, Philippe; Moppert, X; Guezennec, Jean.
Data: | 2008-08
Ano: | 2008
Palavras-chave: | Polyhydroxyalkanaote; PHAs; Pseudomonas; "kopara"; Microbial mat.
Resumo: | Pseudomonasguezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52 h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change during the fermentation process. However, yield of PHAs production varied from 4% of the cellular dry weight (CDW) to 63% obtained after 36 h. Scan electron microscopy was used to study the morphology and organization of PHAs granules within the cells and revealed the presence of several granules occupying almost the entire cell volume. (C) 2008 Elsevier B.V. All rights reserved.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2008/publication-6119.pdf

DOI:10.1016/j.ijbiomac.2008.04.011

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/6119/
Formato: | application/pdf
Fonte: | International Journal of Biological Macromolecules (0141-8130) (Elsevier), 2008-08 , Vol. 43 , N. 2 , P. 176-181
Fonte: | 2008 Elsevier B.V. All rights reserved.