Data/hora: 11/06/2024 10:48:58

Provedor de dados: 	5	
País: 	France	
Título: 	Vibrio aestuarianus zinc metalloprotease causes lethality in the Pacific oyster Crassostrea gigas and impairs the host cellular immune defenses	
Autores: 	Labreuche, Yannick; Le Roux, Frederique; Henry, Joel; Zatylny, Celine; Huvet, Arnaud; Lambert, Christophe; Soudant, Philippe; Mazel, Didier; Nicolas, Jean-louis.	
Data: 	2010-11	
Ano: 	2010	
Palavras-chave: 	Vibrio aestuarianus; Metalloprotease; Crassostrea gigas; Oyster; Hemocytes; Extracellular products.	
Resumo: 	Extracellular products (ECPs) of the pathogenic Vibrio aestuarianus 01/32 were previously reported to display lethality in Crassostrea gigas oysters and to cause morphological changes and immunosuppression in oyster hemocytes. To identify the source of this toxicity, biochemical and genetic approaches were developed. ECP protease activity and lethality were shown to be significantly reduced following incubation with metal chelators, suggesting the involvement of a zinc metalloprotease. An open reading frame of 1836 bp encoding a 611-aa metalloprotease (designated yam) was identified. The deduced protein sequence showed high homology to other Vibrio metalloproteases reported to be involved in pathogenicity. To further confirm the role of this enzyme in ECP toxicity, a plasmid carrying the yarn gene under the control of an araC-P-BAD expression cassette was transferred to a Vibrio splendidus related strain, LMC20012(T), previously characterized as non-pathogenic to oysters. Expression of Vam conferred a toxic phenotype to LMG20012(T) ECPs in vivo and cytotoxicity to oyster hemocytes in vitro. Collectively, these data suggest that the Vam metalloprotease is a major contributor to the toxicity induced by V aestuarianus ECPs and is involved in the impairment of oyster hemocyte functions. (C) 2010 Elsevier Ltd. All rights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00015/12649/9588.pdf

DOI:10.1016/j.fsi.2010.07.007	
Editor: 	Academic Press Ltd- Elsevier Science Ltd	
Relação: 	http://archimer.ifremer.fr/doc/00015/12649/	
Formato: 	application/pdf	
Fonte: 	Fish &amp; Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2010-11 , Vol. 29 , N. 5 , P. 753-758	
Fonte: 	2010 Elsevier Ltd All rights reserved.