Data/hora: 11/06/2024 09:10:12

Provedor de dados: | 5
País: | France
Título: | Optimization of common cuttlefish (Sepia officinalis) protein hydrolysate using Pepsin by Response Surface Methodology
Autores: | Kechaou, Emna; Berge, Jean-pascal; Jaouen, Pascal; Ben Amar, Raja.
Data: | 2015-04
Ano: | 2015
Palavras-chave: | Cuttlefish protein hydrolysate (CPH); Amino acid; Enzyme; Sepia officinalis.
Resumo: | Protein hydrolysate was prepared from common cuttlefish Sepia officinalis by Pepsin. Hydrolysis conditions (time, temperature, and enzyme activity) were optimized by response surface methodology (RSM) using a factorial design. The regression coefficient was close to 0.999, observed during both experimental and validation runs, and indicated the validity of prediction model. An enzyme activity to substrate ratio (X1), 0.04 AU/g protein; time (X2), 85 minutes; and temperature (X3), 45 °C were found to be the optimum conditions for a higher degree of hydrolysis (21%) and nitrogen recovery (90%) using Pepsin. According to amino acid analysis results and chemical score, methionine and histidine are the limiting amino acids in the hydrolysates, in that order. All other amino acids are present in sufficient quantity as required.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00182/29285/27715.pdf

DOI:10.1080/10498850.2013.773116

http://archimer.ifremer.fr/doc/00182/29285/

Editor: | Taylor &amp; Francis Inc
Formato: | application/pdf
Fonte: | Journal Of Aquatic Food Product Technology (1049-8850) (Taylor &amp; Francis Inc), 2015-04 , Vol. 24 , N. 3 , P. 270-282

Fonte:

Taylor &amp; Francis Group, LLC

info:eu-repo/semantics/openAccess

restricted use