Data/hora: 06/06/2024 12:59:57

Provedor de dados: | 5
País: | France
Título: | Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
Autores: | Hogrel, Gaelle; Lu, Yang; Laurent, Sebastien; Henry, Etienne; Etienne, Clarisse; Duy Khanh Phung,; Dulermo, Remi; Bosse, Audrey; Pluchon, Pierre Francois; Clouet-d'Orval, Beatrice; Flament, Didier.
Data: | 2018-06
Ano: | 2018
Resumo: | Several archaeal species prevalent in extreme environments are particularly exposed to factors likely to cause DNA damages. These include hyperthermophilic archaea (HA), living at temperatures &gt;70°C, which arguably have efficient strategies and robust genome guardians to repair DNA damage threatening their genome integrity. In contrast to Eukarya and other archaea, homologous recombination appears to be a vital pathway in HA, and the Mre11–Rad50 complex exerts a broad influence on the initiation of this DNA damage response process. In a previous study, we identified a physical association between the Proliferating Cell Nuclear Antigen (PCNA) and the Mre11–Rad50 (MR) complex. Here, by performing co-immunoprecipitation and SPR analyses, we identified a short motif in the C- terminal portion of Pyrococcus furiosus Mre11 involved in the interaction with PCNA. Through this work, we revealed a PCNA-interaction motif corresponding to a variation on the PIP motif theme which is conserved among Mre11 sequences of Thermococcale species. Additionally, we demonstrated functional interplay in vitro between P. furiosus PCNA and MR enzymatic functions in the DNA end resection process. At physiological ionic strength, PCNA stimulates MR nuclease activities for DNA end resection and promotes an endonucleolytic incision proximal to the 5′ strand of double strand DNA break.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00439/55092/56534.pdf

http://archimer.ifremer.fr/doc/00439/55092/56535.pdf

DOI:10.1093/nar/gky322

http://archimer.ifremer.fr/doc/00439/55092/

Editor: | Oxford Univ Press
Formato: | application/pdf
Fonte: | Nucleic Acids Research (0305-1048) (Oxford Univ Press), 2018-06 , Vol. 46 , N. 11 , P. 5651-5663

Fonte:

The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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