Data/hora: 10/06/2024 18:14:16

Provedor de dados: 	5	
País: 	France	
Título: 	Dynamic epimarks in sex-related genes predict gonad phenotype in the European sea bass, a fish with mixed genetic and environmental sex determination	
Autores: 	Anastasiadi, Dafni; Vandeputte, Marc; Sanchez-baizan, Nuria; Allal, Francois; Piferrer, Francesc.	
Data: 	2018	
Ano: 	2018	
Palavras-chave: 	Early development; Environmental temperature; DNA methylation; Sex determination; Aromatase; Cyp19a1a; Dmrt1; Epigenetic marks; DNA methylation threshold; Epigenetic inheritance.	
Resumo: 	The integration of genomic and environmental influences into methylation patterns to bring about a phenotype is of central interest in developmental epigenetics, but many details are still unclear. The sex ratios of the species used here, the European sea bass, are determined by genetic and temperature influences. We created four families from parents known to produce offspring with different sex ratios, exposed larvae to masculinizing temperatures and examined, in juvenile gonads, the DNA methylation of seven genes related to sexual development by a targeted sequencing approach. The genes most affected by both genetics and environment were cyp19a1a and dmrt1, with contrasting sex-specific methylation and temperature responses. The relationship between cyp19a1a methylation and expression is relevant to the epigenetic regulation of vertebrate sex, and we report the evidence of such relationship only below a methylation threshold, similar to 80%, and that it was sex-specific: negatively correlated in females but positively correlated in males. From parents to offspring, the methylation in gonads was midway between oocytes and sperm, with bias towards oocytes for amh-r2, er-beta 2, fsh-r and cyp19a1a. In contrast, dmrt1 levels resembled those of sperm. The methylation of individual CpGs from foxl2, er-beta 2 and nr3c1 were conserved from parents to offspring, whereas those of cyp19a1a, dmrt1 and amh-r2 were affected by temperature. Utilizing a machine-learning procedure based on the methylation levels of a selected set of CpGs, we present the first, to our knowledge, system based on epigenetic marks capable of predicting sex in an animal with similar to 90% accuracy and discuss possible applications.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00470/58123/61657.pdf

DOI:10.1080/15592294.2018.1529504

https://archimer.ifremer.fr/doc/00470/58123/	
Editor: 	Taylor &amp; Francis Inc	
Relação: 	info:eu-repo/grantAgreement/EC/FP7/262336/EU//AQUA EXCEL	
Formato: 	application/pdf	
Fonte: 	Epigenetics (1559-2294) (Taylor &amp; Francis Inc), 2018 , Vol. 13 , N. 9 , P. 988-1011	
Fonte: 	info:eu-repo/semantics/openAccess

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