Data/hora: 06/06/2024 15:48:52

Provedor de dados: 	5	
País: 	France	
Título: 	Optimisation of growth conditions for continuous culture of the hyperthermophilic archaeon Thermococcus hydrothermalis and development of sulphur-free defined and minimal media	
Autores: 	Postec, Anne; Pignet, Patricia; Cueff-gauchard, Valerie; Schmitt, Anne; Querellou, Joel; Godfroy, Anne.	
Data: 	2005	
Ano: 	2005	
Palavras-chave: 	Bioreactor; Defined and minimal media; Culture optimisation; Gas lift bioreactor; Thermococcus hydrothermalis.	
Resumo: 	The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80degreesC, pH 6 and gas sparging at 0.2 v v(-1) min(-1). Higher steady state cell densities were obtained on a medium containing maltose and yeast extract. In order to design a defined and minimal media, the nutritional requirements of T hydrothermalis were then investigated using continuous culture in the absence of elemental sulphur in the gas-lift bioreactor. First, the complex nutriments were replaced and a defined medium containing maltose, 19 amino acids and the two nitrogenous bases adenine and thymine, was determined. Secondly, selective feedings and withdrawal of amino acids showed requirements for 14 amino acids.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2005/publication-365.pdf

DOI:10.1016/j.resmic.2004.08.001	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/365/	
Formato: 	application/pdf	
Fonte: 	Research in Microbiology (0923-2508) (Elsevier), 2005 , Vol. 156 , N. 1 , P. 82-87	
Fonte: 	2004 Elsevier SAS All rights reserved