Data/hora: 14/06/2024 12:22:56

Provedor de dados: 	5	
País: 	France	
Título: 	Fluorescent antibody enumeration of Vibrio cholerae in the marine environment.	
Autores: 	Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D.	
Data: 	1984-10	
Ano: 	1984	
Palavras-chave: 	Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection.	
Resumo: 	From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for environmental studies, a more realistic, i.e., valid estimate of V. cholerae O1 population size can be obtained directly from environmental samples.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/1984/acte-1000.pdf	
Editor: 	Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986	
Relação: 	http://archimer.ifremer.fr/doc/00000/1000/	
Formato: 	application/pdf