Data/hora: 07/06/2024 05:23:30

Provedor de dados: | 5
País: | France
Título: | Fluorescent antibody enumeration of Vibrio cholerae in the marine environment.
Autores: | Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D.
Data: | 1984-10
Ano: | 1984
Palavras-chave: | Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection.
Resumo: | From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for environmental studies, a more realistic, i.e., valid estimate of V. cholerae O1 population size can be obtained directly from environmental samples.
Tipo: | Text
Idioma: | Inglês
Identificador: | http://archimer.ifremer.fr/doc/1984/acte-1000.pdf
Editor: | Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986
Relação: | http://archimer.ifremer.fr/doc/00000/1000/
Formato: | application/pdf