Data/hora: 10/06/2024 12:05:31

Provedor de dados: 	5	
País: 	France	
Título: 	Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts	
Autores: 	Estevez, Pablo; Sibat, Manoella; Leão-martins, José Manuel; Reis Costa, Pedro; Gago-martínez, Ana; Hess, Philipp.	
Data: 	2020-04	
Ano: 	2020	
Palavras-chave: 	Ciguatoxins; HRMS; Q-TOF; Ciguatera poisoning; C-CTX1; Fragmentation pathways.	
Resumo: 	Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00624/73630/73067.pdf

https://archimer.ifremer.fr/doc/00624/73630/73068.pdf

DOI:10.3390/toxins12040267

https://archimer.ifremer.fr/doc/00624/73630/	
Editor: 	MDPI AG	
Formato: 	application/pdf	
Fonte: 	Toxins (2072-6651) (MDPI AG), 2020-04 , Vol. 12 , N. 4 , P. 267 (7p.)	
Fonte: 	info:eu-repo/semantics/openAccess

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