Data/hora: 14/06/2024 09:18:06

Provedor de dados: 	5	
País: 	France	
Título: 	Caractérisation et analyse de l'expression des pénaeidines, peptides antimicrobiens isolés chez la crevette pénéide Penaeus vannamei (Crustacea, Decapoda)	
Autores: 	Destoumieux, Delphine.	
Data: 	1998-12-10	
Ano: 	1998	
Palavras-chave: 	Crustacé; Crevette; Immunité; Hémocyte; Peptide; Antimicrobien; Cationique.	
Resumo: 	Haemocytes are the key elements of a complex defence system on which crustacean immunity is largely based. We report here for the first time in a crustacean that haemocytes are involved in the synthesis of antimicrobial peptides similar to those described in other arthropod classes. These molecules isolated from the penaeid shrimp Penaeus vannamei are members of an homogeneous farnily which differ from the antimicrobial peptides previously described in that they combine in a single molecule a proline-rich arninoterminal domain, and a cyclic carboxy-terminal domain containing three intramolecular disulphide bridges. In addition, penaeidins bear post-translational modifications at their arnino- and carboxy-termini. Five molecules of the penaeidin family were isolated by a biochemical approach and by cDNA cloning. They are characterised by antibacterial properties essentially directed against Gram positive strains et by antifungal activities against filamentous fungi. Penaeidins are mainly synthesised by haemocytes where they are stored within the cytoplasmic granules of the so-called granular and semigranular cell types. They are also present in haemocyte containing tissues (heart, lymphoid organ), in intestinal epithelium, and in gonads. Lower  expression levels were detected in hepatopancreas. From our results, and in opposition to the insect immune system, it seems that penaeidin expression is not transcriptionnally regulated upon infection, but that the bioactive peptides are released by haemocyte exocytosis in response to bacterial challenge. These data strongly suggest that antimicrobial peptide expression in crustacean is regulated in a similar manner to that described in chelicerata.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	http://archimer.ifremer.fr/doc/00440/55111/56564.pdf

http://archimer.ifremer.fr/doc/00440/55111/	
Editor: 	Université de Montpellier II	
Formato: 	application/pdf	
Fonte: 	1998 Université de Montpellier II

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