Data/hora: 14/06/2024 19:29:45

Provedor de dados: | 5
País: | France
Título: | Detection of griseofulvin in a marine strain of Penicillium waksmanii by ion trap mass spectrometry
Autores: | Petit, Karina; Mondeguer, Florence; Roquebert, M; Biard, J; Pouchus, Y.
Data: | 2004-07
Ano: | 2004
Palavras-chave: | Mass spectrometry ESI IT/MS; Griseofulvin; Antifungal; Marine fungi; Penicillium waksmanii.
Resumo: | A marine strain of Penicillium waksmanii Zaleski was isolated from a sample of seawater from shellfish-farming area in the Loire estuary (France). The in vitro marine culture showed an important antifungal activity. Bioassay-guided fractionation was used to purify the crude extract. Dereplication by electrospray-ion trap/mass spectrometry (ESI-IT/MS) afforded the identification of the antifungal compound, after a semi-purification consisting of two stages. A comparison of the ionic composition between the active and the non-active fractions allowed the detection of a monocharged ion at m/z 353 containing a chlorine atom, which could be attributed to the antifungal griseofulvin [C17H17ClO6+H](+). Multi-stage fragmentation (MSn) confirmed the identity of the m/z 353 ion of the antifungal fraction as griseofulvin. It is the first description of griseofulvin production by a strain of P. waksmanii and the first chemical study of a strain of this species isolated from marine temperate cold water.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2004/publication-673.pdf

DOI:10.1016/j.mimet.2004.03.004

Editor: | Elsevier
Relação: | http://archimer.ifremer.fr/doc/00000/673/
Formato: | application/pdf
Fonte: | Journal of Microbiological Methods (0167-7012) (Elsevier), 2004-07 , Vol. 58 , N. 1 , P. 59-65
Fonte: | 2004 Elsevier B.V. All rights reserved