Data/hora: 01/06/2024 00:47:32

Provedor de dados: 	5	
País: 	France	
Título: 	Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps	
Autores: 	Vigneron, Adrien; L Haridon, Stephane; Godfroy, Anne; Roussel, Erwan; Cragg, Barry A.; Parkes, R. John; Toffin, Laurent.	
Data: 	2015-05	
Ano: 	2015	
Resumo: 	In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various microbial communities, such as abundant lineages of anaerobic methanotrophs (ANME). However, the biodiversity, distribution, and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabeled dimethylamine, bicarbonate, and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of denaturing gradient gel electrophoresis (DGGE) fingerprinting and reverse-transcribed PCR-amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages, confirming the cooccurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00256/36737/35338.pdf

DOI:10.1128/AEM.00147-15

http://archimer.ifremer.fr/doc/00256/36737/	
Editor: 	Amer Soc Microbiology	
Formato: 	application/pdf	
Fonte: 	Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2015-05 , Vol. 81 , N. 10 , P. 3451-3459	
Fonte: 	2015, American Society for Microbiology. All Rights Reserved

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