Data/hora: 18/06/2024 18:00:25

Provedor de dados: 	5	
País: 	France	
Título: 	Osteoprotegerin, a new actor in vasculogenesis, stimulates endothelial colony-forming cells properties	
Autores: 	Benslimane-ahmim, Z.; Heymann, D.; Dizier, B.; Lokajczyk, A.; Brion, R.; Laurendeau, I.; Bieche, I.; Smadja, D. M.; Galy-fauroux, I.; Colliec-jouault, Sylvia; Fischer, Anne-marie; Boisson-vidal, Catherine.	
Data: 	2011-04	
Ano: 	2011	
Palavras-chave: 	Endothelial colony-forming cells; Osteoprotegerin; Receptor activator of nuclear factor-kappa B ligand; Vasculogenesis.	
Resumo: 	Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We found that ECFC expressed OPG and RANK but not RANKL mRNA. Treatment of ECFC with VEGF or stromal cell-derived factor-1 (SDF-1) upregulated OPG mRNA expression. OPG stimulated ECFC migration (P &lt; 0.05), chemotaxis (P &lt; 0.05) and vascular cord formation on Matrigel (R) (P &lt; 0.01). These effects were correlated with SDF-1 mRNA overexpression, which was 30-fold higher after 4 h of OPG stimulation (P &lt; 0.01). OPG-mediated angiogenesis involved the MAPK signaling pathway as well as Akt or mTOR cascades. RANKL also showed pro-vasculogenic effects in vitro. OPG combined with FGF-2 promoted neovessel formation in vivo, whereas RANKL had no effect. Conclusions: OPG induces ECFC activation and is a positive regulator of microvessel formation in vivo. Our results suggest that the OPG/RANK/RANKL axis may be involved in vasculogenesis and strongly support a modulatory role in tissue revascularization.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00033/14462/11863.pdf

DOI:10.1111/j.1538-7836.2011.04207.x	
Editor: 	Wiley-blackwell	
Relação: 	http://archimer.ifremer.fr/doc/00033/14462/	
Formato: 	application/pdf	
Fonte: 	Journal Of Thrombosis And Haemostasis (1538-7933) (Wiley-blackwell), 2011-04 , Vol. 9 , N. 4 , P. 834-843	
Fonte: 	2011 International Society on Thrombosis and Haemostasis, Wiley-Blackwell