Data/hora: 09/06/2024 14:45:57

Provedor de dados: | 5
País: | France
Título: | Osteoprotegerin, a new actor in vasculogenesis, stimulates endothelial colony-forming cells properties
Autores: | Benslimane-ahmim, Z.; Heymann, D.; Dizier, B.; Lokajczyk, A.; Brion, R.; Laurendeau, I.; Bieche, I.; Smadja, D. M.; Galy-fauroux, I.; Colliec-jouault, Sylvia; Fischer, Anne-marie; Boisson-vidal, Catherine.
Data: | 2011-04
Ano: | 2011
Palavras-chave: | Endothelial colony-forming cells; Osteoprotegerin; Receptor activator of nuclear factor-kappa B ligand; Vasculogenesis.
Resumo: | Background: Osteoprotegerin (OPG), a soluble receptor of the tumour necrosis factor family, and its ligand, the receptor activator of nuclear factor-kappa B ligand (RANKL), are emerging as important regulators of vascular pathophysiology. Objectives: We evaluated their effects on vasculogenesis induced by endothelial colony-forming cells (ECFC) and on neovessel formation in vivo. Methods: Effects of OPG and RANKL on in vitro angiogenesis were evaluated after ECFC incubation with OPG or RANKL (0-50 ng mL(-1)). Effects on microvessel formation were evaluated with an in vivo murin Matrigel plug assay. Vascularization was evaluated by measuring plug hemoglobin and vascular endothelial growth factor (VEGF)-R2 content 14 days after implantation. Results: We found that ECFC expressed OPG and RANK but not RANKL mRNA. Treatment of ECFC with VEGF or stromal cell-derived factor-1 (SDF-1) upregulated OPG mRNA expression. OPG stimulated ECFC migration (P &lt; 0.05), chemotaxis (P &lt; 0.05) and vascular cord formation on Matrigel (R) (P &lt; 0.01). These effects were correlated with SDF-1 mRNA overexpression, which was 30-fold higher after 4 h of OPG stimulation (P &lt; 0.01). OPG-mediated angiogenesis involved the MAPK signaling pathway as well as Akt or mTOR cascades. RANKL also showed pro-vasculogenic effects in vitro. OPG combined with FGF-2 promoted neovessel formation in vivo, whereas RANKL had no effect. Conclusions: OPG induces ECFC activation and is a positive regulator of microvessel formation in vivo. Our results suggest that the OPG/RANK/RANKL axis may be involved in vasculogenesis and strongly support a modulatory role in tissue revascularization.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/00033/14462/11863.pdf

DOI:10.1111/j.1538-7836.2011.04207.x

Editor: | Wiley-blackwell
Relação: | http://archimer.ifremer.fr/doc/00033/14462/
Formato: | application/pdf
Fonte: | Journal Of Thrombosis And Haemostasis (1538-7933) (Wiley-blackwell), 2011-04 , Vol. 9 , N. 4 , P. 834-843
Fonte: | 2011 International Society on Thrombosis and Haemostasis, Wiley-Blackwell