Data/hora: 06/06/2024 06:04:44

Provedor de dados: 	5	
País: 	France	
Título: 	First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system	
Autores: 	De Decker, Sophie; Reynaud, Yann; Saulnier, Denis.	
Data: 	2013-05	
Ano: 	2013	
Palavras-chave: 	Quorum sensing; Crassostrea gigas; Vibrio; Metalloprotease; Gene expression.	
Resumo: 	The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependent metalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam were performed. A relative increase in expressionwas observed for vsm and, particularly, vam in the presence of the conditioned medium, probably controlled by a quorumsensing system. The study revealed intraspecific and interspecific modulation of expression in metalloprotease genes vsm and vam in V. splendidus and V. aestuarianus. Our experiments showed for the first time that V. splendidus is able to produce an autoinducer-like substance that displays intra- and interspecific effects on the expression of two different metalloprotease genes: vsm and vam	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00124/23539/21381.pdf

DOI:10.1016/j.aquaculture.2013.01.033	
Editor: 	Elsevier Science Bv	
Relação: 	http://archimer.ifremer.fr/doc/00124/23539/	
Formato: 	application/pdf	
Fonte: 	Aquaculture (0044-8486) (Elsevier Science Bv), 2013-05 , Vol. 392 , P. 1-7	
Fonte: 	2013 Elsevier B.V. All rights reserved