Data/hora: 06/06/2024 09:51:50

Provedor de dados: 	5	
País: 	France	
Título: 	Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication	
Autores: 	Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.	
Data: 	2017-10-18	
Ano: 	2017	
Palavras-chave: 	Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.	
Resumo: 	Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters before being challenged by OsHV-1. Survival appeared close to 100% in both dsRNA-injected conditions associated with a low detection of viral DNA and a low expression of a panel of 39 OsHV-1 genes as compared with infected control. Long dsRNA molecules, both Cg-I kappa B2- and GFP-dsRNA, may have induced an anti-viral state controlling the OsHV-1 replication and precluding the understanding of the specific role of Cg-I kappa B2. Immune-related genes including Cg-I kappa B1, Cg-Rel1, Cg-IFI44, Cg-PKR and Cg-IAP appeared activated in the dsRNA-injected condition, potentially hampering viral replication and thus conferring a better resistance to OsHV-1 infection. We revealed that long dsRNA-mediated genetic interference triggered an anti-viral state in the oyster, emphasizing the need for new reverse genetics tools for assessing immune gene function and avoiding off-target effects in bivalves.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00409/52011/52698.pdf

DOI:10.1242/jeb.156299

https://archimer.ifremer.fr/doc/00409/52011/	
Editor: 	Company Of Biologists Ltd	
Relação: 	info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED	
Formato: 	application/pdf	
Fonte: 	Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2017-10-18 , Vol. 220 , N. 20 , P. 3671-3685	
Fonte: 	2017. Published by The Company of Biologists Ltd

info:eu-repo/semantics/openAccess

restricted use