Data/hora: 11/06/2024 01:39:39

Provedor de dados: | 5
País: | France
Título: | Essai de cryoconservation des gamétophytes de l'algue alimentaire Undaria pinnatifida (Laminariale)
Autores: | Arbault, Suzanne; Renard, Philippe; Pérez, Rene; Kaas, Raymond.
Data: | 1990-07
Ano: | 1990
Palavras-chave: | Algue; Cryoconservation; Gamétophyte; Undaria pinnatifida; Alga; Cryopreservation; Gametophyte; Undaria pinnatifida.
Resumo: | Low temperature tolerance was determined for gametophytes of food alga Undaria pinnatifida according to various cryopreservation methods. Survival durations after storage in a medium containing 28% glycerol at −80 °C or in a 5-10% glycerol solution at −196 °C were only 9 and 4 days respectively. On the other hand, cooling at 5 °C/min. and storage at −15 °C for 15 minutes, without cryoprotective medium, did not reduce viability but this was highly decreased by storage for 15 minutes at −30 or −196 °C, with or without DMSO. The morphological cooling injuries were depigmentation and/or a contraction of the cells.
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00189/30071/28559.pdf

DOI:10.1051/alr:1990021

Editor: | EDP Sciences
Relação: | http://archimer.ifremer.fr/doc/00189/30071/
Formato: | application/pdf
Fonte: | Aquatic Living Resources (0990-7440) (EDP Sciences), 1990-07 , Vol. 3 , N. 3 , P. 207-215
Fonte: | IFREMER-Gauthier-Villars, 1990