Data/hora: 10/06/2024 11:09:44

Provedor de dados: 	5	
País: 	France	
Título: 	Essai de cryoconservation des gamétophytes de l'algue alimentaire Undaria pinnatifida (Laminariale)	
Autores: 	Arbault, Suzanne; Renard, Philippe; Pérez, Rene; Kaas, Raymond.	
Data: 	1990-07	
Ano: 	1990	
Palavras-chave: 	Algue; Cryoconservation; Gamétophyte; Undaria pinnatifida; Alga; Cryopreservation; Gametophyte; Undaria pinnatifida.	
Resumo: 	Low temperature tolerance was determined for gametophytes of food alga Undaria pinnatifida according to various cryopreservation methods. Survival durations after storage in a medium containing 28% glycerol at −80 °C or in a 5-10% glycerol solution at −196 °C were only 9 and 4 days respectively. On the other hand, cooling at 5 °C/min. and storage at −15 °C for 15 minutes, without cryoprotective medium, did not reduce viability but this was highly decreased by storage for 15 minutes at −30 or −196 °C, with or without DMSO. The morphological cooling injuries were depigmentation and/or a contraction of the cells.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	http://archimer.ifremer.fr/doc/00189/30071/28559.pdf

DOI:10.1051/alr:1990021	
Editor: 	EDP Sciences	
Relação: 	http://archimer.ifremer.fr/doc/00189/30071/	
Formato: 	application/pdf	
Fonte: 	Aquatic Living Resources (0990-7440) (EDP Sciences), 1990-07 , Vol. 3 , N. 3 , P. 207-215	
Fonte: 	IFREMER-Gauthier-Villars, 1990