Data/hora: 11/06/2024 03:46:35

Provedor de dados: 	5	
País: 	France	
Título: 	Vibrio tapetis, the Causative Agent of Brown Ring Disease, Forms Biofilms with Spherical Components	
Autores: 	Rodrigues, Sophie; Paillard, Christine; Le Pennec, Gael; Dufour, Alain; Bazire, Alexis.	
Data: 	2015-12	
Ano: 	2015	
Palavras-chave: 	Vibrio tapetis; Biofilm; Brown Ring Disease; Ruditapes philippinarum; Spherical components; Temperature effect; Salinity effect.	
Resumo: 	Vibrio tapetis is a marine bacterium causing Brown Ring Disease (BRD) in the Manila clam Ruditapes philippinarum. V. tapetis biofilm formation remains unexplored depite the fact that it might be linked to pathogenicity. Our objectives were to characterize the in vitro biofilm formation of V tapetis and evaluate the effects of culture conditions. Biofilm structure and its matrix composition were examined by confocal laser scanning microscopy and scanning electron microscopy. V tapetis was able to form biofilms on a glass substratum within 24 h. Polysaccharides and extracellular DNA of the biofilm matrixes were differently distributed depending on the V tapetis strains. Spherical components of about 1-2 mu m diameter were found at the biofilm surface. They contain DNA, proteins, and seemed to be physically linked to bacteria and of cellular nature. Transmission electron microscopy showed that the spherical components were devoid of internal compartments. Temperatures &gt;21 degrees C inhibit BRD whereas low salinity (2%) favor it, none of the both conditions altered V tapetis' ability to form biofilms in vitro. We suggest therefore that biofilm formation could play a role in the persistence of the pathogen in clam than in BRD symptoms.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00307/41783/42796.pdf

https://archimer.ifremer.fr/doc/00307/41783/42804.mov

https://archimer.ifremer.fr/doc/00307/41783/42805.mov

DOI:10.3389/fmicb.2015.01384

https://archimer.ifremer.fr/doc/00307/41783/	
Editor: 	Frontiers Media Sa	
Formato: 	application/pdf	
Fonte: 	Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2015-12 , Vol. 6 , N. 1384 , P. 1-10	
Fonte: 	2015 Rodrigues, Paillard, Le Pennec, Dufour and Bazire. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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