Data/hora: 06/06/2024 11:54:24

Provedor de dados: 	5	
País: 	France	
Título: 	Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli?	
Autores: 	Allain, Thibault; Chaouch, Soraya; Thomas, Myriam; Travers, Marie-agnes; Valle, Isabelle; Langella, Philippe; Grellier, Philippe; Polack, Bruno; Florent, Isabelle; Bermudez-humaran, Luis G.	
Data: 	2018-02	
Ano: 	2018	
Palavras-chave: 	Giardia duodenalis; Lactobacilli; Lactobacillus johnsonii; Lactobacillus gasseri; Probiotics; Bile salt hydrolases.	
Resumo: 	Giardia duodenalis is a protozoan parasite responsible for giardiasis, a disease characterized by intestinal malabsorption, diarrhea and abdominal pain in a large number of mammal species. Giardiasis is one of the most common intestinal parasitic diseases in the world and thus a high veterinary, and public health concern. It is wellestablished that some probiotic bacteria may confer protection against this parasite in vitro and in vivo and we recently documented the implication of bile-salt hydrolase (BSH)-like activities from strain La1 of Lactobacillus johnsonii as mediators of these effects in vitro. We showed that these activities were able to generate deconjugated bile salts that were toxic to the parasite. In the present study, a wide collection of lactobacilli strains from different ecological origins was screened to assay their antigiardial effects. Our results revealed that the anti-parasitic effects of some of the strains tested were well-correlated with the expression of BSH-like activities. The two most active strains in vitro, La1 and Lactobacillus gasseri  CNCM I-4884, were then tested for their capacity to influence G. duodenalis infection in a suckling mice model. Strikingly, only L. gasseri CNCM I-4884 strain was able to significantly antagonize parasite growth with a dramatic reduction of the trophozoites load in the small intestine. Moreover, this strain also significantly reduced the fecal excretion of Giardia cysts after 5 days of treatment, which could contribute to blocking the transmission of the parasite, in contrast of La1 where no effect was observed. This study represents a step toward the development of new prophylactic strategies to combat G. duodenalis infection in both humans and animals	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	https://archimer.ifremer.fr/doc/00424/53569/54417.pdf

https://archimer.ifremer.fr/doc/00424/53569/54418.tiff

https://archimer.ifremer.fr/doc/00424/53569/54419.tiff

DOI:10.3389/fmicb.2018.00089

https://archimer.ifremer.fr/doc/00424/53569/	
Editor: 	Frontiers Media Sa	
Formato: 	application/pdf	
Fonte: 	Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2018-02 , Vol. 9 , N. 89 , P. 9p.	
Fonte: 	2018 Allain, Chaouch, Thomas, Travers, Valle, Langella, Grellier, Polack, Florent and Bermúdez-Humarán. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice.

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