Data/hora: 11/06/2024 03:41:27

Provedor de dados: | 5
País: | France
Título: | Flow cytometric assessment of morphology, viability, and production of reactive oxygen species of Crassostrea gigas oocytes. Application to Toxic dinoflagellate (Alexandrium minutum) exposure
Autores: | Le Goic, Nelly; Hegaret, Helene; Boulais, Myrina; Beguel, Jean-philippe; Lambert, Christophe; Fabioux, Caroline; Soudant, Philippe.
Data: | 2014-12
Ano: | 2014
Palavras-chave: | Pacific oyster; Gamete quality; ROS production; Viability; Flow cytometry; Ecotoxicology.
Resumo: | The Pacific oyster Crassostrea gigas accounts for a large part of shellfish aquaculture production worldwide. Aspects of morphological and functional characteristics of oyster oocytes remain poorly documented, and traditional techniques, such as microscopic observations of shape or fertilization rate, are time and space consuming. The purpose of this study was to assess for the first time viability and reactive oxygen species (ROS) production of Pacific oyster oocytes using flow cytometry (FCM) and to apply this method to determine oocyte responses to in vitro exposure to the toxic dinoflagellate Alexandrium minutum. A culture of A. minutum caused a significant increase in oocyte ROS production, which gradually increased with the age of the culture, but viability was not affected. Effect of the supernatant of the same A. minutum culture did not cause any significant modifications of oocyte morphology, viability, or ROS level. This study confirmed that some oocyte cellular characteristics can be assessed using FCM techniques.
Tipo: | Text
Idioma: | Inglês

Identificador:

https://archimer.ifremer.fr/doc/00216/32760/31321.pdf

DOI:10.1002/cyto.a.22577

https://archimer.ifremer.fr/doc/00216/32760/

Editor: | Wiley-blackwell
Formato: | application/pdf
Fonte: | Cytometry Part A (1552-4922) (Wiley-blackwell), 2014-12 , Vol. 85A , N. 12 , P. 1049-1056

Fonte:

2014 International Society for Advancement of Cytometry

info:eu-repo/semantics/openAccess

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