Data/hora: 06/06/2024 07:52:48

Provedor de dados: 	5	
País: 	France	
Título: 	Etude in vivo et in vitro des effets du mercure sur la réponse hémocytaire de l'huître creuse, Crassostrea gigas : développement de techniques évaluant les impacts de la pollution estuarienne	
Autores: 	Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.	
Data: 	2002-09-12	
Ano: 	2002	
Palavras-chave: 	Flow cytometry; Haemocytes; Mercury; Crassostrea gigas; Cytométrie de flux; Hémocytes; Mercure; Crassostrea gigas.	
Resumo: 	In the last decades, shellfish industry represents an important economic activity in France and particularly in Charente-Maritime. Marennes-Oleron basin is the first French area of oyster production, especially Pacific  oyster, Crassostrea gigas. But this area is also subjected to many pollution due to anthropic activities. Industrial wastes are sources of mercury pollution in the Charente river. The harmful effects of such contaminants on animals inhabiting these estuarine zones are poorly known, although many studies begin to be developed. Most of these studies use bivalve molluscs as biological indicators : these animals can bioaccumulate pollutants within their tissues by filtration. Moreover, they are sedentary so they cannot escape in the case of a chronic or sudden pollution.   In this context, influence of mercury on defence mechanisms developed by Pacific oyster, Crassostrea gigas, was studied. Mercury effects were analysed in vitro on haemocytes, immune cells in bivalves, and also in vivo in laboratory on adult oysters. Effects of this pollutant on haemocyte activities have been monitored.  For all experiments, haemocytes were analysed by flow cytometry ; this technique allows to monitor different haemocyte characters in a short period. Viability and cellular activities have been analysed.  Results indicate that mercury induces an in vivo oyster mortality and an in vitro high haemocyte mortality.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2002/acte-830.pdf	
Editor: 	V° Congrès International de Limnologie-Océanographie, 9-12 septembre, Paris - 28 (1-2) : 34-38.	
Relação: 	http://archimer.ifremer.fr/doc/00000/830/	
Formato: 	application/pdf