Data/hora: 09/06/2024 08:37:13

Provedor de dados: | 5
País: | France
Título: | Dérivés de l'exopolysaccharide d’Alteromonas infernus, mimétiques de glycosaminoglycanes, et développement d'une stratégie pour leur analyse structurale
Autores: | Akoumany, Katy.
Data: | 2019-11-15
Ano: | 2019
Palavras-chave: | Bactérie marine; Exopolysaccharides; Dépolymérisation enzymatique; Synthèse organique; Marine bacteria; Exopolysaccharides; Enzymatic depolymerisation; Organic synthesis.
Resumo: | In search of new bioactive compounds, marine bacteria constitute a considerable source of innovative molecules. The bacterial exopolysaccharide (EPS), produced by the deep-sea hydrothermal vent strain Alteromonas infernus, is a high-molecular-weight, highly branched and anionic heteropolysaccharide with a nonasaccharide repeating unit. This macromolecule and its low-molecular-weight derivatives obtained through a chemical process have previously displayed interesting GAG-like properties such as anti-metastatic and anti-coagulant (heparin-like) ones; they can also improve stem cell differentiation. To investigate the molecular bases of the biological activity and to facilitate the structural analysis of the bioactive derivatives (~20 000 g/mol), fragments of the native EPS molecule have been prepared by organic synthesis and enzymatic depolymerisation. The first part of this thesis is dedicated to the synthesis of the disaccharides composing the repeating unit of the EPS. The synthetic pathways to acceptor and donor glycosyls have been developed and two protected disaccharides have been synthetized.The second part is devoted to the preparation of oligosaccharides and low-molecular-weight derivatives by enzymatic depolymerization of the native EPS. A new generation of low-molecular-weight derivatives has been produced and a new bisulfated octasaccharide has been characterized by mass spectrometry.
Tipo: | Text
Idioma: | Francês

Identificador:

https://archimer.ifremer.fr/doc/00605/71733/70206.pdf

https://archimer.ifremer.fr/doc/00605/71733/

Editor: | Université de Nantes
Formato: | application/pdf

Fonte:

info:eu-repo/semantics/openAccess

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