Data/hora: 11/06/2024 13:25:26

Provedor de dados: 	5	
País: 	France	
Título: 	Métabolisme et interactions bactériennes en lien avec la vitamine B12 chez la microalgue haptophyte Tisochrysis lutea	
Autores: 	Nef, Charlotte.	
Data: 	2019-11-13	
Ano: 	2019	
Palavras-chave: 	Métabolisme; Vitamine B12; Interactions microbiennes; Microalgues haptophytes; Metabolism; Vitamin B12; Microbial interactions; Haptophyte microalgae.	
Resumo: 	Vitamin B12 is an enzymatic cofactor that enables methionine synthesis and whose availability can control phytoplankton development in marine systems. This vitamin is synthesized only by select species of bacteria and archaea. Haptophyte microalgae form a widespread group with major ecological importance. However, little is known about their utilization of vitamin B12 nor their interactions with B12 producers. Our main objectives were to characterize mechanisms of acquisition and utilization of vitamin B12 by Haptophytes and the effect of B12 limitation on their metabolism by considering the model species Tisochrysis lutea. Analysis of the repartition of B12 dependency in haptophytes by molecular approaches suggest that this group would be the first to gather only B12-dependent species. We characterized physiologic plasticity of T. lutea depending on B12 availability by coupling phenotypic and proteomics analyses. Vitamin B12 limitation impacts methionine and central carbon metabolisms as well as synthesis of major osmolytes including DMSP. Furthermore, our results suggest that T. lutea does not excrete key organic compounds that would allow the establishment of mutualistic relationship with B12-producing bacteria. The microalgae is however able to assimilate bacterial lysate enriched with B12, indicating an indirect acquisition of the vitamin. This work is the first to analyse B12 molecular physiology of haptophyte microalgae in B12-limited environments and to investigate the link between these microalgae and B12 producers.	
Tipo: 	Text	
Idioma: 	Francês	
Identificador: 	https://archimer.ifremer.fr/doc/00598/71003/69268.pdf

https://archimer.ifremer.fr/doc/00598/71003/	
Editor: 	Université de Nantes	
Formato: 	application/pdf	
Fonte: 	info:eu-repo/semantics/openAccess

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