Data/hora: 10/06/2024 12:17:47

Provedor de dados: | 5
País: | France
Título: | Association among growth, food consumption-related traits and amylase gene polymorphism in the Pacific oyster Crassostrea gigas
Autores: | Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane.
Data: | 2008-12
Ano: | 2008
Palavras-chave: | Ingestion; Gene expression; Bivalve; Alpha amylase.
Resumo: | To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to ingestion than to absorption efficiency. Additionally, relative mRNA levels of the two amylase cDNAs were also strongly associated with amylase gene polymorphism, possibly reflecting variation in an undefined regulatory region, although no corresponding variation was observed in specific amylase activity. Amylase gene sequences were determined for each genotype, showing the existence of only synonymous or functionally equivalent non-synonymous polymorphisms. The observed associations among growth, food consumption-related traits and amylase gene polymorphism are therefore more likely to be related to variation in the level of amylase gene expression than to functional enzymatic variants.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2008/publication-5165.pdf

DOI:10.1111/j.1365-2052.2008.01776.x

Editor: | Blackwell science
Relação: | http://archimer.ifremer.fr/doc/00000/5165/
Formato: | application/pdf
Fonte: | Animal Genetics (0268-9146) (Blackwell science), 2008-12 , Vol. 39 , N. 6 , P. 662-665
Fonte: | 2008 Blackwell science